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Centre research facility

The research facility contains three setups equipped with optical tweezers. One setup has holographic optical tweezers for force measurements (Lab 2) and one setup is combined with microfluidics (Lab 1). Two of the labs are also equipped and suitable for experiments with mammalian cells (Lab 1 and Lab 3). The facility also has two preparation labs for biological, e.g. yeast and algae (Lab 4) and chemical samples (Lab 5), respectively, as well as a cleanroom facility for mask production and PDMS work to construct advanced microfluidic systems (Lab 6). In close proximity to these labs are also a sterile cell culturing facility, with all the equipment necessary for mammalian cell culturing (Lab 7). New users must first attend an introductory course before using any of the equipment. The introduction contains an overview of how the equipment work and a review of safety regulations.

Contact information

Mattias Goksör (manager) +46 31-786 9125
Caroline Beck Adiels (responsible for the labs) +46 31-786 9123

Lab 1 (F3307a)

The setup is currently used for experiments with yeast, mammalian cells or algae and combine optical trapping and microfluidics. Imaging is performed using a Leica DMI6000B epi-fluorescence microscope equipped with an EM-CCD camera. A single, stationary optical trap is produced using a 1070 nm Ytterbium fiber laser. For microfluidics experiments, mechanical syringe pumps alternatively, pressure driven pumps, are used.

To the same microscope a spinning disk unit is coupled. Two lasers (488 nm and 405 nm) can be used for simultaneous imaging and create confocal-like z-stacks without the limitations of confocal microscopy imaging.

Lab 2 (F3307b)

The setup is used for holographic optical force measurements and has been used measuring on yeast cells. Imaging is performed using a Leica DMI4000i inverted microscope equipped with a high-speed CMOS camera for particle tracking and a second camera for sample monitoring. Holographic optical tweezers are produced using a 1070 nm Ytterbium fiber laser and a spatial light modulator.

Lab 3 (F3314)

The setup is used for single cell force experiments using optical manipulation. Imaging is performed using a Leica DMI4000B epi-fluorescence microscope equipped with a high-speed camera. A single optical tweezers which can be moved in three dimensions is produced using a 1070 nm Ytterbium fiber laser, a gimbal mounted mirror and a lens translator. Particle tracking can be performed by either using the high-speed camera or using a probe laser and a position sensitive detector. An incubator controlling gas and temperature enable experiments with mammalian cells. Using a second -large field of view- camera (Andor, Zyla sCMOS), this setup is also used for long-term mammalian cell migration studies with fluorescent visualization.

Lab 4 (F3309)

The bio prep lab is equipped with a spectrophotometer, a pH meter, incubator, one shaker incubator, bench microcentrifuge, sonicator, Gene pulser, PCR machine (MJ Mini, BioRad), tempered bench centrifuge, a -80C freezer as well as an ordinary fridge and freezer.

Lab 5 (F3322)

The chemistry lab has a UV-Vis-NIR spectrophotometer (Cary 500 Scan, Varian), Millipore Synthesis A10 (Milli-Q), an autoclave, chemical hood, ultrasonic cleaner, hot plates and scales.

Lab 6 (F6311)

The PDMS and mask making clean room have a Safety Cabinet for spinning resists and mask aligning. The equipment for making masks consists of a WS-400BH-NPP-Lite Single Wafer Spin Processor, two hot plates, a home made mask aligner, 2 fume hoods for developing and molding as well as a microscope for analyzing the result.
Working with PDMS includes a vacuum reservoir, scales and an oven. A plasma cleaner for surface treatment is also available.

This Mini-infrastructure is available free of use for University of Gothenburg and Chalmers staff. Contact Caroline Beck Adiels for more information.

Lab 7 (F3320)

Since autumn 2015, there is also a sterile mammalian cell culture facility available in close proximity to the other labs. In this room, work only with mammalian cell lines, and human stem cells are allowed. The equipment is made up by a Biological Safety Cabinet for laminar flow bench work, two incubators, a vacuum reservoir for easy fluid handling, and a microscope. A small fridge and freezer is also available.

Contact Information

Caroline Beck Adiels

Origovägen 6B, 412 96 Göteborg

Visiting Address:
Office S1043, Soliden

Phone:
46 (0)31 786 9123

Page Manager: Måns Henningson|Last update: 5/12/2016
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